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RNA is prepared from cells, reverse transcribed to cDNA, and labelled with fluorescent dyes .
Green dye for normal and red dye is for cancer cells.
The fluorescent probes are mixed and hybridized to a cDNA array.
Each spot on the array is an oligonucleotide (or cDNA fragment) that represents a different gene.
Image is captured by fluorescence camera where:
a) Red spot indicates higher expression in tumor cells.
b) Green spot indicates lower expression in tumor cells.
c) Yellow spot indicates equal expression level in both normal and tumor samples
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