Blood Banking

BLOOD- BANKING
SAGM additive solution provides
optimum red Cell viability
1) Sodium Chloride provides isotonicity
2) Adenine maintains ATP for red cell
viability
3) Glucose supports red cell metabolism
4) Mannitol helps reduce red cell lysis
• Shelf life (whole blood / red cells ) in :
1) ACD( acidified citrate dextrose) = 21
days
(> 70% transfused cells viable after 24
hours)
2) CPD( Citrate phosphate dextrose) = 28
days
3) Citrate Phosphate Dextrose Adenine
(CPDA) = 35 days
4) SAGM ( Saline Adenine Glucose
Mannitol)= 42 days
• Whole blood:
Storage temp.= 2-6 0c
1 unit raises Hb by ---1gm/dl and
haematocrit by 3%.
After collection from donor ,blood should
be processed for component separation
within 6 hrs
• Storage and duration
1) Whole blood / packed red cells--- 2-6
0c for 42 days
2) Platelets----22-24 0c for 3 days with
continuous agitation
3) Fresh frozen plasma---below -25 0c
for 1 year
4) Cryoprecipitate------ below -25 0c for
1 year
• Transfusion protocols:
Transfusion should commence within 30
minutes of removing blood bag from
refrigerator of blood bag.
(after that it will increase risk of bacterial
contamination)
1) Whole blood /packed rbc --
transfusion must be completed within 4
hrs.
2) Platelet and FFP----within 20
minutes.
Transfusion set should have standard
filter of 170 micron meter pore size
Usual needle size =18-19 gauge
Not necessary to warm blood before
transfusion
Patient should be monitored during 1st
15 minutes, following this every hour, at
the end and after 4 hrs after end of
transfusion.