Banding

•Types of banding in cytogenetics

▪G-banding - obtained with Giemsa stain following digestion of chromosome with trypsin. The dark regions tend to be heterochromatic, late replicating AT rich and inactive. The light regions tend to be euchromatic , early replicating and GC rich

▪R-banding - reverse of G-banding(R stands for reverse)

▪C-banding - stains Centromeres

▪Q-banding - fluoresecent pattern obtained using quinacrine for staining. Pattern of bands is very similar to that seen in G-banding

▪T-banding - visualize telomeres